11955-1-AP

antibody from Proteintech Group

Targeting: HELLS LSH, Nbla10143, PASG, SMARCA6

Provider product page for 11955-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [18]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 11955-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11955-1-AP, RRID:AB_2117529
Product name: HELLS antibody
Antibody type: Polyclonal
Description: HELLS antibody (Cat. #11955-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

HELLS protein structure - 11955-1-AP shown in red.

Submitted references HELLS inhibits autophagy‑dependent ferroptosis in nasopharyngeal carcinoma by modulating the Nrf2/HO‑1/GPX4 pathway.

Jin C, Li J, Han S, Bai C, Yang J, Wang Z
International journal of molecular medicine 2026 May;57(5)

Photoclick chemistry led to the identification of HELLS as a helicase for DNA G-quadruplexes.

Gao Z, Chong Y, He X, Yuan J, Wang Y
Nucleic acids research 2026 Jan 22;54(3)

Multiple Epigenetic Mechanisms Functionally Cooperate to Silence Expression of Somatostatin Receptor Type 2 in Pancreatic Neuroendocrine Tumors.

Madigan JP, Andrews SG, Farrell RB, Forsythe SD, Sharma R, Ceribelli M, Thomas CJ, Shamsian KN, Lin S, Cheng KC, Sadowski SM
bioRxiv : the preprint server for biology 2025 Sep 25;

DNA methylation influences human centromere positioning and function.

Salinas-Luypaert C, Dubocanin D, Lee RJ, Andrade Ruiz L, Gamba R, Grison M, Velikovsky L, Angrisani A, Scelfo A, Xu Y, Dumont M, Barra V, Wilhelm T, Velasco G, Losito M, Wardenaar R, Francastel C, Foijer F, Kops GJPL, Miga KH, Altemose N, Fachinetti D
Nature genetics 2025 Oct;57(10):2509-2521

Dual roles of USP1 in HELLS deubiquitination and SUMOylation drive EMT and FOLFOX-based chemoresistance.

Gao J, Bai N, Liu M, Yao N, Tang Z, Xu B, Chen W, He X, Shao J, Bian S, Zhao H, Zheng W
Oncogenesis 2025 Dec 22;14(1):49

The ZBTB24-CDCA7-HELLS axis suppresses the totipotent 2C-like reprogramming by maintaining Dux methylation and repression.

Guo D, Du Z, Liu Y, Lin M, Lu Y, Hardikar S, Xue Y, Zhang J, Chen T, Dan J
Nucleic acids research 2025 Apr 10;53(7)

Protocadherin 17 weakens the lenvatinib resistance of liver cancer through inducing ferroptosis.

Yang J, Hu B, Zhang G, Wu K, Zhang X, Ji M, Zhang B, Shi H, Li D
Experimental cell research 2025 Apr 1;447(1):114495

The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS.

Shinkai A, Hashimoto H, Shimura C, Fujimoto H, Fukuda K, Horikoshi N, Okano M, Niwa H, Debler EW, Kurumizaka H, Shinkai Y
Nucleic acids research 2024 Sep 23;52(17):10194-10219

CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses.

Vukic M, Chouaref J, Della Chiara V, Dogan S, Ratner F, Hogenboom JZM, Epp TA, Chawengsaksophak K, Vonk KKD, Breukel C, Ariyurek Y, San Leon Granado D, Kloet SL, Daxinger L
Science advances 2024 Feb 9;10(6):eadk3384

Immunological function and prognostic value of lymphoid-specific helicase in liver hepatocellular carcinoma.

Fang Y, Tang W, Zhao D, Zhang X, Li N, Yang Y, Jin L, Li Z, Wei B, Miao Y, Zeng Z, Huang H
Cancer biomarkers : section A of Disease markers 2023;38(2):225-239

Multi-Omics Analysis Reveals Translational Landscapes and Regulations in Mouse and Human Oocyte Aging.

Huang J, Chen P, Jia L, Li T, Yang X, Liang Q, Zeng Y, Liu J, Wu T, Hu W, Kee K, Zeng H, Liang X, Zhou C
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Sep;10(26):e2301538

Germinal center output is sustained by HELLS-dependent DNA-methylation-maintenance in B cells.

Cousu C, Mulot E, De Smet A, Formichetti S, Lecoeuche D, Ren J, Muegge K, Boulard M, Weill JC, Reynaud CA, Storck S
Nature communications 2023 Sep 14;14(1):5695

The histone chaperone ANP32B regulates chromatin incorporation of the atypical human histone variant macroH2A.

Mandemaker IK, Fessler E, Corujo D, Kotthoff C, Wegerer A, Rouillon C, Buschbeck M, Jae LT, Mattiroli F, Ladurner AG
Cell reports 2023 Oct 31;42(10):113300

HELLS serves as a poor prognostic biomarker and its downregulation reserves the malignant phenotype in pancreatic cancer.

Wang FJ, Jing YH, Cheng CS, Cao ZQ, Jiao JY, Chen Z
BMC medical genomics 2021 Jul 27;14(1):189

Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes.

Dybas JM, Lum KK, Kulej K, Reyes ED, Lauman R, Charman M, Purman CE, Steinbock RT, Grams N, Price AM, Mendoza L, Garcia BA, Weitzman MD
mSystems 2021 Aug 31;6(4):101128msystems0046821

Lymphoid-specific helicase promotes the growth and invasion of hepatocellular carcinoma by transcriptional regulation of centromere protein F expression.

Yang X, Miao BS, Wei CY, Dong RZ, Gao PT, Zhang XY, Lu JC, Gao C, Wang XY, Sun HC, Zhou J, Fan J, Ke AW, Shi GM, Cai JB
Cancer science 2019 Jul;110(7):2133-2144

Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals.

Wu H, Thijssen PE, de Klerk E, Vonk KK, Wang J, den Hamer B, Aytekin C, van der Maarel SM, Daxinger L
Human molecular genetics 2016 Sep 15;25(18):4041-4051

Lsh regulates LTR retrotransposon repression independently of Dnmt3b function.

Dunican DS, Cruickshanks HA, Suzuki M, Semple CA, Davey T, Arceci RJ, Greally J, Adams IR, Meehan RR
Genome biology 2013 Dec 24;14(12):R146

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP(HELLS antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using HELLS antibody 11955-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human prostate cancer using 11955-1-AP(HELLS antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue