Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-76833 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HELLS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HELLS was performed by loading whole cell lysate in 1X LDS sample buffer with 2-ME from 5 x 10^5 mouse transformed pre-B cells. Protein Ladder was Spectra™ Multicolor High Range Protein Ladder (Product # 26625). Samples were loaded onto a 3-8% Tris-acetate polyacrylamide gel (Product # EA03785BOX). Proteins were transferred to nitrocellulose membrane with wet/tank transfer. Membrane was blocked in 5% milk/TBST. The membrane was probe with a polyclonal anti-HELLS antibody (Product # PA5-76833) at a dilution of 1:1000 in 2% milk/TBST at 4°C overnight, followed by a secondary antibody HRP-anti-rabbit at a dilution of 1:5000 at room temperature for 1 hour. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # PI-32209).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HELLS. Samples were incubated with HELLS polyclonal antibody (Product # PA5-76833).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HELLS in extracts from normal (control) and HELLS knockout (KO) HeLa cells. Samples were incubated with HELLS polyclonal antibody (Product # PA5-76833).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HELLS in paraffin-embedded mouse testis. Samples were incubated with HELLS polyclonal antibody (Product # PA5-76833).