Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- AP52028PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52028PU-N, RRID:AB_11148151
- Product name
- anti SMARCA6 / HELLS (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 488-518 amino acids from the Central region of Human SMARCA6 / HELLS
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of HELLS (arrow) using SMARCA6 / HELLS Antibody (Center) Cat.-No AP52028PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HELLS gene.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of SMARCA6 / HELLS Antibody (Center) Cat.-No AP52028PU-N in mouse Neuro-2a cell line lysates (35ug/lane). This demonstrates the HELLS antibody detected the HELLS protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Neuro-2a cells using SMARCA6 / HELLS Antibody (Center) Cat.-No AP52028PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.