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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP9258c - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP9258c, RRID:AB_10613753
- Product name
- MEF2A Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.465 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of MEF2A Antibody (Center) (Cat. #AP9258c) in mouse brain tissue lysates (35ug/lane). MEF2A (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of MEF2A Antibody (Center)(Cat. #AP9258c) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- MEF2A Antibody (Center) (Cat. #AP9258c) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MEF2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50~100
