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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX129156 - Provider product page
- Provider
- GeneTex
- Product name
- SIN3A antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.
Li W, Zhang Z, Liu X, Cheng X, Zhang Y, Han X, Zhang Y, Liu S, Yang J, Xu B, He L, Sun L, Liang J, Shang Y
The Journal of clinical investigation 2017 Sep 1;127(9):3421-3440
The Journal of clinical investigation 2017 Sep 1;127(9):3421-3440
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- SIN3A antibody detects SIN3A protein by western blot analysis. A 293T whole cell lysate/extractB A431 whole cell lysate/extractC HeLa whole cell lysate/extractD HepG2 whole cell lysate/extract5% SDS-PAGESIN3A antibody (GTX129156) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with SIN3A antibody (GTX129156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SIN3A antibody detects SIN3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SIN3A protein stained by SIN3A antibody (GTX129156) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- SIN3A antibody immunoprecipitates SIN3A protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 £gg whole cell lysate/extract of SIN3A protein expressing 293T cells B : Control with 3 £gg of pre-immune rabbit IgG C : Immunoprecipitation of SIN3A by 3 £gg of SIN3A antibody (GTX129156) 5% SDS-PAGE The immunoprecipitated SIN3A protein was detected by SIN3A antibody (GTX129156) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SIN3A antibody detects SIN3A protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. SIN3A antibody (GTX129156) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SIN3A antibody detects SIN3A protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. SIN3A antibody (GTX129156) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SIN3A antibody detects SIN3A protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SIN3A antibody (GTX129156) dilution: 1:500.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Cross-linked ChIP was performed with MCF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 £gg of either control rabbit IgG or anti-SIN3A antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or MyoD.
