PA5-85554

antibody from Invitrogen Antibodies

Targeting: SIN3A DKFZP434K2235, KIAA0700

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85554

Provider

Invitrogen Antibodies

Product name

SIN3A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution. Predicted reactivity: Mouse (96%), Rat (94%), Chicken (86%), Rhesus Monkey (100%). Positive Control: 293T, A431, HeLa, HepG2, mouse brain, rat brain. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.38 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SIN3A in 4% paraformaldehyde-fixed HeLa cells using SIN3A polyclonal antibody (Product # PA5-85554) at a dilution of 1:1000. Sample was then incubated with Hoechst secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SIN3A Polyclonal Antibody detects SIN3A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3A stained by SIN3A Polyclonal Antibody (Product # PA5-85554) diluted at 1:1,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SIN3A in 4% paraformaldehyde-fixed HeLa cells using SIN3A polyclonal antibody (Product # PA5-85554) at a dilution of 1:1000. Sample was then incubated with Hoechst secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SIN3A Polyclonal Antibody detects SIN3A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3A stained by SIN3A Polyclonal Antibody (Product # PA5-85554) diluted at 1:1,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of SIN3A in A) 293T whole cell lysate of SIN3A protein expressing 293T cells, B) control of pre-immune rabbit IgG, C) immunoprecipitation of SIN3A protein with SIN3A polyclonal antibody (Product # PA5-85554) using A) 30 µg, B)3 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SIN3A Polyclonal Antibody immunoprecipitates SIN3A protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of SIN3A protein expressing 293T cells B : Control with 3 µg of pre-immune rabbit IgG C : Immunoprecipitation of SIN3A by 3 µg of SIN3A Polyclonal Antibody (Product # PA5-85554) 5% SDS-PAGE The immunoprecipitated SIN3A protein was detected by SIN3A Polyclonal Antibody (Product # PA5-85554) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SIN3A Polyclonal Antibody detects SIN3A protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SIN3A Polyclonal Antibody (Product # PA5-85554) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SIN3A in paraffin-embedded mouse prostate using SIN3A polyclonal antibody (Product # PA5-85554) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SIN3A in paraffin-embedded mouse colon using SIN3A polyclonal antibody (Product # PA5-85554) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SIN3A Polyclonal Antibody detects SIN3A protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. SIN3A Polyclonal Antibody (Product # PA5-85554) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SIN3A in paraffin-embedded rat fore brain using SIN3A polyclonal antibody (Product # PA5-85554) at a dilution of 1:500.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cross-linked ChIP was performed with MCF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 µg of either control rabbit IgG or SIN3A Polyclonal Antibody (Product # PA5-85554). The precipitated DNA was detected by PCR with primer set targeting to GAPDH or MyoD.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cross-linked ChIP was performed with MCF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 µg of either control rabbit IgG or SIN3A Polyclonal Antibody (Product # PA5-85554). The precipitated DNA was detected by PCR with primer set targeting to GAPDH or MyoD.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP (chromatin immunoprecipitation) analysis of SIN3A in MCF-7 chromatin extract with SIN3A polyclonal antibody (Product # PA5-85554) using 5 µg of sample.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of SIN3A in A) 293T whole cell lysate of SIN3A protein expressing 293T cells, B) control of pre-immune rabbit IgG, C) immunoprecipitation of SIN3A protein with SIN3A polyclonal antibody (Product # PA5-85554) using A) 30 µg, B)3 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SIN3A Polyclonal Antibody immunoprecipitates SIN3A protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of SIN3A protein expressing 293T cells B : Control with 3 µg of pre-immune rabbit IgG C : Immunoprecipitation of SIN3A by 3 µg of SIN3A Polyclonal Antibody (Product # PA5-85554) 5% SDS-PAGE The immunoprecipitated SIN3A protein was detected by SIN3A Polyclonal Antibody (Product # PA5-85554) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.