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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NB100-2253 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2253, RRID:AB_2187629
- Product name
- Rabbit Polyclonal SIN3A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SIN3A Antibody [NB100-2253] - Detection of Human Sin3A by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Sin3A antibody NB100-2253 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SIN3A Antibody [NB100-2253] - Detection of Human and Mouse Sin3A on HeLa whole cell lysate using NB100-2253. Sin3A was also immunoprecipitated using rabbit anti-Sin3A antibodies NB100-2252, NB100-2254 and another Sin3A antibody.
