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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- 102-PA23 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- NRP-1
- Antibody type
- Polyclonal
- Antigen
- Recombinant human NRP-1
- Description
- antibody Protein-A purified from serum
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEW
LIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFD
GENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSD
YETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSP
GFPEKYPNSLECTYIVFAPKMSEIILEFESFDLEP
DSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQK
TPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQ
SSVSEDFKCMEALGMESGEIHSDQITASSQYSTNW
SAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFV
TAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWIT
IKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRI
KPATWETGISMRFEVYGCKITDYPCSGMLGMVSGL
ISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPA
PHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVF
MRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNY
DTPELRTFPALSTRFIRIYPERATHGGLGLRMELL
GCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGD
DFQLTGGTTVLATEKPTVIDSTIQSGIKLEHHHHH
H- Antibody clone number
- Rabbit IG
- Vial size
- 200 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Analysis of anti-human NRP-1. Sample was loaded in 10% SDS-polyacrylamide gel under non-reducing conditions.
- Sample type
- Purified recombinant protein
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining (green) of Neuropilin-1 (NRP-1) in primary human umbilical vein endothelial cells (HUVEC) with anti-human NRP-1 (1µg/ml) [Cat# 102-PA23] and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 (Dianova) was used 1:800.
- Sample type
- HUVEC
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with primary human umbilical vein endothelial cells (HUVEC).
