Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 102-PA23AG - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- NRP-1
- Antibody type
- Polyclonal
- Antigen
- Recombinant human NRP-1
- Description
- antibody Antigen-affinity purified from serum
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEW
LIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFD
GENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSD
YETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSP
GFPEKYPNSLECTYIVFAPKMSEIILEFESFDLEP
DSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQK
TPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQ
SSVSEDFKCMEALGMESGEIHSDQITASSQYSTNW
SAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFV
TAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWIT
IKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRI
KPATWETGISMRFEVYGCKITDYPCSGMLGMVSGL
ISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPA
PHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVF
MRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNY
DTPELRTFPALSTRFIRIYPERATHGGLGLRMELL
GCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGD
DFQLTGGTTVLATEKPTVIDSTIQSGIKLEHHHHH
H- Antibody clone number
- Rabbit IG
- Vial size
- 50 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Analysis of anti-human NRP-1. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions. Lane 1: sNRP-1 conditioned supernatant from Sf9 cells. There is a strong cross reactivity with recombinant human sFLT4-Fc chimera but not with the other tested soluble receptors.
- Sample type
- Purified recombinant proteins
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Functional ELISA with anti-human NRP-1. Recombinant human soluble NRP-1 was coated with increasing amounts on a 96 well microtiter plate.
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with primary human umbilical vein endothelial cells (HUVEC).
