AF3870

antibody from Novus Biologicals

Targeting: NRP1 CD304, NRP, VEGF165R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF3870

Provider

Novus Biologicals

Product name

Sheep Polyclonal Neuropilin-1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross-reactivity with recombinant rat Neuropilin-1 and recombinant mouse Neuropilin-1 is observed, and less than 1% cross-reactivity with recombinant human Neuropilin-2 is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

iRGD synergizes with PD-1 knockout immunotherapy by enhancing lymphocyte infiltration in gastric cancer.

Ding N, Zou Z, Sha H, Su S, Qian H, Meng F, Chen F, Du S, Zhou S, Chen H, Zhang L, Yang J, Wei J, Liu B
Nature communications 2019 Mar 22;10(1):1336

---

Cerebral organoids at the air-liquid interface generate diverse nerve tracts with functional output.

Giandomenico SL, Mierau SB, Gibbons GM, Wenger LMD, Masullo L, Sit T, Sutcliffe M, Boulanger J, Tripodi M, Derivery E, Paulsen O, Lakatos A, Lancaster MA
Nature neuroscience 2019 Apr;22(4):669-679

---

Neuropilin-1 mediates neutrophil elastase uptake and cross-presentation in breast cancer cells.

Kerros C, Tripathi SC, Zha D, Mehrens JM, Sergeeva A, Philips AV, Qiao N, Peters HL, Katayama H, Sukhumalchandra P, Ruisaard KE, Perakis AA, St John LS, Lu S, Mittendorf EA, Clise-Dwyer K, Herrmann AC, Alatrash G, Toniatti C, Hanash SM, Ma Q, Molldrem JJ
The Journal of biological chemistry 2017 Jun 16;292(24):10295-10305

---

Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.

Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perrière N, Guillevic O, Couraud PO, Uzan G
PloS one 2014;9(1):e84179

---

Conformational remodeling of the fibronectin matrix selectively regulates VEGF signaling.

Ambesi A, McKeown-Longo PJ
Journal of cell science 2014 Sep 1;127(Pt 17):3805-16

---

Neuropilin-1 mediates PDGF stimulation of vascular smooth muscle cell migration and signalling via p130Cas.

Pellet-Many C, Frankel P, Evans IM, Herzog B, Jünemann-Ramírez M, Zachary IC
The Biochemical journal 2011 May 1;435(3):609-18

---

Alphav beta3 integrin limits the contribution of neuropilin-1 to vascular endothelial growth factor-induced angiogenesis.

Robinson SD, Reynolds LE, Kostourou V, Reynolds AR, da Silva RG, Tavora B, Baker M, Marshall JF, Hodivala-Dilke KM
The Journal of biological chemistry 2009 Dec 4;284(49):33966-81

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Neuropilin-1 by Western Blot. Detection of Human Neuropilin-1 by Western Blot.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Neuropilin-1 in Human Pancreatic Cancer Tissue. Neuropilin-1 in Human Pancreatic Cancer Tissue.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Neuropilin-1 in HUVEC Human Cells by Flow Cytometry. Detection of Neuropilin-1 in HUVEC Human Cells by Flow Cytometry.