34-7300

antibody from Invitrogen Antibodies

Targeting: NRP1 CD304, NRP, VEGF165R

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

34-7300

Provider

Invitrogen Antibodies

Product name

Anti-Neuropilin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.25 mg/ml

Storage

-20°C

References

Submitted references

Neuropilin 1 Receptor Is Up-Regulated in Dysplastic Epithelium and Oral Squamous Cell Carcinoma.

Shahrabi-Farahani S, Gallottini M, Martins F, Li E, Mudge DR, Nakayama H, Hida K, Panigrahy D, D'Amore PA, Bielenberg DR
The American journal of pathology 2016 Apr;186(4):1055-64

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Prognostic implication of neuropilin-1 upregulation in human nasopharyngeal carcinoma.

Xu Y, Li P, Zhang X, Wang J, Gu D, Wang Y
Diagnostic pathology 2013 Sep 20;8:155

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Downregulation of neuropilin-1 in patients with acute myeloid leukemia treated with thalidomide.

Kreuter M, Steins M, Woelke K, Buechner T, Berdel WE, Mesters RM
European journal of haematology 2007 Nov;79(5):392-7

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Correlation of neuropilin-1 overexpression to survival in acute myeloid leukemia.

Kreuter M, Woelke K, Bieker R, Schliemann C, Steins M, Buechner T, Berdel WE, Mesters RM
Leukemia 2006 Nov;20(11):1950-4

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Vascular endothelial growth factor induces branching morphogenesis/tubulogenesis in renal epithelial cells in a neuropilin-dependent fashion.

Karihaloo A, Karumanchi SA, Cantley WL, Venkatesha S, Cantley LG, Kale S
Molecular and cellular biology 2005 Sep;25(17):7441-8

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1) and Rat Brain (Lane 2). The blot was probed with Neuropilin 1 Rabbit polyclonal Antibody (Product # 34-7300, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 025 µg/mL, 1:4000 dilution). A 68 kDa band corresponding to Neuropilin 1 was observed across the tissues tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).