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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- FAB5994R-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Neuropilin-1 Alexa Fluor® 647-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Neuropilin-1 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant rat (rr) Neuropilin-1 is observed and no cross-reactivity with recombinant human (rh) Neuropilin-1, rhNeuropilin-2, or rrNeuropilin-2 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Red dye
- Antigen sequence
P97333- Isotype
- IgG
- Antibody clone number
- 761705
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Neuropilin-1 in CD4+ Mouse Splenocytes by Flow Cytometry. CD4+ mouse splenocytes were stained with Rabbit Anti-Human/Mouse FoxP3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC8214G) and either (A) Rat Anti-Mouse Neuropilin-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB5994R) or (B) Rat IgG2A Alexa Fluor 647 Isotype Control (Catalog # IC006R). To facilitate intracellular staining of FoxP3, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Splenocytes were gated on CD4.View our protocol for Staining Membrane-associated Proteins.
