Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX127947 - Provider product page
- Provider
- GeneTex
- Product name
- Neuropilin 1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Mouse, Rat
- Host
- Rabbit
Submitted references Smooth muscle cell-specific knockout of neuropilin-1 impairs postnatal lung development and pathological vascular smooth muscle cell accumulation.
Mahmoud M, Evans IM, Mehta V, Pellet-Many C, Paliashvili K, Zachary I
American journal of physiology. Cell physiology 2019 Mar 1;316(3):C424-C433
American journal of physiology. Cell physiology 2019 Mar 1;316(3):C424-C433
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuropilin 1 antibody (GTX127947) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuropilin 1 antibody (GTX127947) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuropilin 1 antibody (GTX127947) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Neuropilin 1 antibody detects Nrp1 protein by Western blot analysis.A. 50 ?g Rat brain lysate/extract5 % SDS-PAGENeuropilin 1 antibody (GTX127947) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Neuropilin 1 antibody detects Neuropilin 1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: Neuropilin 1 antibody (GTX127947) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).Blue: Nuclear staining with Hoechst 33342.
