PAB12639

antibody from Abnova Corporation

Targeting: NRP1 CD304, NRP, VEGF165R

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12639

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12639, RRID:AB_10554368

Product name

NRP1 (phospho T916) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of NRP1.

Antigen sequence

LNTQS


Storage

Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Neuropilin-1 is a receptor for transforming growth factor beta-1, activates its latent form, and promotes regulatory T cell activity.

Glinka Y, Prud'homme GJ
Journal of leukocyte biology 2008 Jul;84(1):302-10

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Molecular pathways regulating pro-migratory effects of Hedgehog signaling.

Hochman E, Castiel A, Jacob-Hirsch J, Amariglio N, Izraeli S
The Journal of biological chemistry 2006 Nov 10;281(45):33860-70

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The 4 mg protein of whole tissue lysates derived from human brain were immunoprecipitated by 4 ug of NRP1 (phospho T916) polyclonal antibody (Cat # PAB12639) o/n at 4°C, and probed by the same antibody at 1 : 1000. An immunoreactive band at ~ 130kDa was observed.

Validation comment

Immunoprecipitation

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot Blot : 1 ug peptide was blot onto NC membrane.A : NRP1 (Phospho-specific peptide at pT916).B : NRP1 (Non-phospho phosphopeptide).C : Non-related phospho-specific phophopeptide.Followed were blotted at a 1 : 1000 dilution by :1. NRP1 (phospho T916) polyclonal antibody (Cat # PAB12639).2. NRP1 polyclonal antibody (Cat # PAB12638).

Validation comment

Dot Blot (Peptide)