Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 47-3049-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD304 (Neuropilin-1) Monoclonal Antibody (TNKUSOHA), APC-eFluor™ 780, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The TNKUSOHA monoclonal antibody recognizes human CD304, which is also known as BDCA-4 and Neuropilin-1. Like CD303, the CD304 antigen is expressed on human plasmacytoid dendritic cells in normal blood and bone marrow. CD304 can also be detected on T follicular helper cells in inflamed tonsils, as well as on some B-cell acute lymphoblastic leukemias. In addition, CD304 is expressed on numerous non-hematopoietic cell types, including neurons, endothelial, and tumor cells. Plasmacytoid dendritic cells play a key role in the innate immune response against viral pathogens and tumors. Applications Reported: This TNKUSOHA antibody has been reported for use in flow cytometric analysis. Applications Tested: This TNKUSOHA antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- TNKUSOHA
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD123 PE (Product # 12-1239-42) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD304 APC-eFluor® 780 (right). Cells in the monocyte gate were used for analysis.