Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB5640 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal NFATC1/NFAT2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human NFATC1 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references CDK4/6 Inhibition Augments Antitumor Immunity by Enhancing T-cell Activation.
Deng J, Wang ES, Jenkins RW, Li S, Dries R, Yates K, Chhabra S, Huang W, Liu H, Aref AR, Ivanova E, Paweletz CP, Bowden M, Zhou CW, Herter-Sprie GS, Sorrentino JA, Bisi JE, Lizotte PH, Merlino AA, Quinn MM, Bufe LE, Yang A, Zhang Y, Zhang H, Gao P, Chen T, Cavanaugh ME, Rode AJ, Haines E, Roberts PJ, Strum JC, Richards WG, Lorch JH, Parangi S, Gunda V, Boland GM, Bueno R, Palakurthi S, Freeman GJ, Ritz J, Haining WN, Sharpless NE, Arthanari H, Shapiro GI, Barbie DA, Gray NS, Wong KK
Cancer discovery 2018 Feb;8(2):216-233
Cancer discovery 2018 Feb;8(2):216-233
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFATC1/NFAT2 [p Ser172] Antibody (679340) [MAB5640] - Lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 1 um Ionomycin for 24 hours. PVDF Membrane was probed with 1 ug/mL of Human Phospho-NFATC1 (S172) Monoclonal Antibody (R&D Systems catalog # MAB5640) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (R&D Systems catalog # HAF007). A specific band was detected for Phospho-NFATC1 (S172) at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions.