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LearnGTX22796
antibody from GeneTex
Targeting: NFATC1
NF-ATC, NFAT2, NFATc
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Gel shift Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX22796 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX22796, RRID:AB_378022
- Product name
- NFAT2 antibody [7A6]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Hamster, Rabbit, Simian
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NFATc1 in 293 cells. NFATc1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with NFATc1 antibody [7A6] at a dilution of 1:20 over night at 4 ¢XC, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with or without NFATc1 antibody [7A6] overnight at 4¢XC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
