PA5-79731

antibody from Invitrogen Antibodies

Targeting: NFATC1 NF-ATC, NFAT2, NFATc

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-79731

Provider

Invitrogen Antibodies

Product name

Anti-NFATC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The synthetic peptide sequence is 700-716aa, AIFLTVSREHERVGCFF Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

Lot Dependent

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFATC1 in Lane 1: PANC whole cell lysate, Lane 2: U20S whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with NFATC1 polyclonal antibody (Product # PA5-79731) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NFATC1 on Jurkat cells. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with NFATC1 polyclonal antibody (Product# PA5-79731) with a dilution of 2 µg/mL (overnight at 4°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.