Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [4]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- MA5-32686 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFATC1 Recombinant Rabbit Monoclonal Antibody (JA81-03)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JA81-03
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NFATC1 in 293T cells using a NFATC1 Monoclonal antibody (Product # MA5-32686) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NFATC1 in MCF-7 cells using a NFATC1 Monoclonal antibody (Product # MA5-32686) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NFATC1 in 293T cells using a NFATC1 Monoclonal antibody (Product # MA5-32686) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NFATC1 in Hela cells using a NFATC1 Monoclonal antibody (Product # MA5-32686) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NFATC1 of paraffin-embedded Human thymus tissue using a NFATC1 Monoclonal antibody (Product #MA5-32686). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NFATC1 of paraffin-embedded Human tonsil tissue using a NFATC1 Monoclonal antibody (Product #MA5-32686). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NFATC1 of paraffin-embedded Human spleen tissue using a NFATC1 Monoclonal antibody (Product #MA5-32686). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NFATC1 of paraffin-embedded Human skeleton muscle tissue using a NFATC1 Monoclonal antibody (Product #MA5-32686). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of NFATC1 in Jurkat cells using a NFATC1 Monoclonal Antibody (Product # MA5-32686) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).