Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
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Validation data
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- Product number
- NB100-56732 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56732, RRID:AB_838619
- Product name
- Rabbit Polyclonal NFATC1/NFAT2 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified. The amino acid sequence used is 90% homologous in mouse and rat and 100% identical in human NFATc1 A, B, C and E isoforms. The reported amino acid size of NFATc1 isoforms are as follows: A -716 aa, B-825 aa, C-930 aa, and E-812 aa.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references α-Linolenic Acid Inhibits Receptor Activator of NF-κB Ligand Induced (RANKL-Induced) Osteoclastogenesis and Prevents Inflammatory Bone Loss via Downregulation of Nuclear Factor-KappaB-Inducible Nitric Oxide Synthases (NF-κB-iNOS) Signaling Pathways.
NOTCH1 is a mechanosensor in adult arteries.
Song J, Jing Z, Hu W, Yu J, Cui X
Medical science monitor : international medical journal of experimental and clinical research 2017 Oct 24;23:5056-5069
Medical science monitor : international medical journal of experimental and clinical research 2017 Oct 24;23:5056-5069
NOTCH1 is a mechanosensor in adult arteries.
Mack JJ, Mosqueiro TS, Archer BJ, Jones WM, Sunshine H, Faas GC, Briot A, Aragón RL, Su T, Romay MC, McDonald AI, Kuo CH, Lizama CO, Lane TF, Zovein AC, Fang Y, Tarling EJ, de Aguiar Vallim TQ, Navab M, Fogelman AM, Bouchard LS, Iruela-Arispe ML
Nature communications 2017 Nov 20;8(1):1620
Nature communications 2017 Nov 20;8(1):1620
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: NFATC1/NFAT2 Antibody [NB100-56732] - Lane view shows a specific band for NFAT2 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Analysis of NFATc1 in Ramos cell lysate. (1) Without blocking peptide. (2) With blocking peptide.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Analysis of human (A)Ramos and (B) mouse RAW lysate using NFATc1 antibody at 1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Immortalized mouse podocytes. Image from verified customer review.