PA5-27713

antibody from Invitrogen Antibodies

Targeting: MAP3K14 FTDCR1B, HS, HSNIK, NIK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27713

Provider

Invitrogen Antibodies

Product name

NIK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NIK Polyclonal Antibody (Product # PA5-27713). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: Hep G2 . 7.5% SDS PAGE. NIK Polyclonal Antibody (Product # PA5-27713) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using anti-NIK/ MAP3K14 Polyclonal Antibody (Product # PA5-27713) and a 104 kDa band corresponding to NIK/MAP3K14 was observed in Mouse Liver only when compared to other tissues. Uncharacterized bands between 40-60 kDa were also observed in the samples tested. Whole cell extracts (30 µg lysate) of Mouse Liver (Lane1), Rat Liver (Lane 2), Mouse Heart (Lane 3), Rat Heart (Lane 4), Mouse Brain (Lane 5) and Rat Brain (Lane 6) were electrophoresed using Novex® NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).