H00009020-D01P

antibody from Abnova Corporation

Targeting: MAP3K14 FTDCR1B, HS, HSNIK, NIK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009020-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009020-D01P, RRID:AB_1677226

Product name

MAP3K14 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human MAP3K14 protein.

Antigen sequence

MAVMEMACPGAPGSAVGQQKELPKAKEKTPPLGKK
QSSVYKLEAVEKSPVFCGKWEILNDVITKGTAKEG
SEAGPAAISIIAQAECENSQEFSPTFSERIFIAGS
KQYSQSESLDQIPNNVAHATEGKMARVCWKGKRRS
KARKKRKKKSSKSLAHAGVALAKPLPRTPEQESCT
IPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQLCF
KQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHP
QDGGPLPLPTHPFPYSRLPHPFPFHPLQPWKPHPL
ESFLGKLACVDSQKPLPDPHLSKLACVDSPKPLPG
PHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQA
HSLTSLAKTWAARGSRSREPSPKTEDNEGVLLTEK
LKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDK
QTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPL
YGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPED
RALYYLGQALEGLEYLHSRRILHGDVKADNVLLSS
DGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTE
THMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHP
WTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAI
QEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPW
RGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGP
RPAEETTGRAPKLQPPLPPEPPEPNKSPPLTLSKE
ESGMWEPLPLSSLEPAPARNPSSPERKATVPEQEL
QQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSL
SDDSEKNPSKASQSSRDTLSSGVHSWSSQAEARSS
SWNMVLARGRPTDTPSYFNGVKVQIQSLNGEHLHI
REFHRVKVGDIATGISSQIPAAAFSLVTKDGQPVR
YDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLEN
RP


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MAP3K14 expression in transfected 293T cell line (H00009020-T02) by MAP3K14 MaxPab polyclonal antibody.Lane 1: MAP3K14 transfected lysate(104.00 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to MAP3K14 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between MAP3K14 and CHUK. HeLa cells were stained with anti-MAP3K14 rabbit purified polyclonal 1:1200 and anti-CHUK mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)