PA5-100732

antibody from Invitrogen Antibodies

Targeting: MAP3K14 FTDCR1B, HS, HSNIK, NIK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-100732

Provider

Invitrogen Antibodies

Product name

NIK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total NIK.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B.

Vallecillo-Zúniga ML, Poulson PD, Luddington JS, Arnold CJ, Rathgeber M, Kartchner BC, Hayes S, Gill H, Valdoz JC, Spallino JL, Garfield S, Dodson EL, Arthur CM, Stowell SR, Van Ry PM
Cells 2021 Nov 17;10(11)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NIK in 3T3 cell lysate (left lane: treated with blocking peptide). Samples were incubated with NIK polyclonal antibody (Product # PA5-100732).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NIK in Ant NIK-transfected 293 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with NIK polyclonal antibody (Product # PA5-100732).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 In vitro treatment with rHsGal-1 modulates inflammatory response through the NF-kappaB pathway. ( A ) Quantification of expression levels of p65 (normalized to beta-tubulin) in 48 h A/J -/- NT or 0.11 muM rHsGal-1 treated myotubes. ( B ) Western blot images showing the p65 expression in NT or 0.11 muM rHsGal-1 48 h A/J -/- treated myotubes. ( C ) Representative images of WT and NT or 0.11 muM rHsGal-1 48 h A/J -/- treated myotubes cultured and immunostained with p65 (green), Phalloidin (red), and DAPI (blue). ( D - H ) Western blot quantification of 48 h NT or 0.11 muM rHsGal-1treated myotubes expressing levels of TAK1 ( D ), NIK ( E ), IKBalpha ( F ), p50 ( G ), and P-p65 ( H ). ( I ) Western blot images of 48 h NT or 0.11 muM rHsGal-1treated myotubes expressing NF-kappaB inflammatory subunits quantified in D-H. n = 3 in each group. A. * p < 0.05 and ** p < 0.01 NT vs. all forms of Gal-1. D-H. * p < 0.05, ** p < 0.01, *** p < 0.001 NT vs. rHsGal-1. Data are represented as SEM.