Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
Reference
Comment
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- Product number
- LS-B2365 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B2365, RRID:AB_2167927
- Product name
- IHC-plus™ NCF1 / p47phox / p47 phox Antibody (aa378-390) LS-B2365
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Bovine, Simian
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Submitted references NADPH oxidase expression in active multiple sclerosis lesions in relation to oxidative tissue damage and mitochondrial injury.
Fischer MT, Sharma R, Lim JL, Haider L, Frischer JM, Drexhage J, Mahad D, Bradl M, van Horssen J, Lassmann H
Brain : a journal of neurology 2012 Mar;135(Pt 3):886-99
Brain : a journal of neurology 2012 Mar;135(Pt 3):886-99
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NCF1 / p47phox antibody (0.2ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NCF1 / p47phox antibody (0.2µg/ml) staining of U937 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NCF1 / p47phox antibody (1µg/ml) staining of Mouse Thymus (A) and Pig Spleen (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.