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- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-38777 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-p47phox (Ser328) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Shear and Integrin Outside-In Signaling Activate NADPH-Oxidase 2 to Promote Platelet Activation.
Xu Z, Liang Y, Delaney MK, Zhang Y, Kim K, Li J, Bai Y, Cho J, Ushio-Fukai M, Cheng N, Du X
Arteriosclerosis, thrombosis, and vascular biology 2021 May 5;41(5):1638-1653
Arteriosclerosis, thrombosis, and vascular biology 2021 May 5;41(5):1638-1653
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-p47phox/NCF1 pSer328 in paraffin-embedded human lymph node tissue using a Phospho-p47phox/NCF1 pSer328 polyclonal antibody (Product # PA5-38777).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6. Differential roles of SFKs (Src family kinases) and Syk in agonist-induced p47phox phosphorylation. A and B , Washed mouse platelets were treated with the SFK inhibitor PP2 (10 umol/L) or negative control compound PP3(10 umol/L), and stimulated with ( A ) 0.025 U/mL thrombin or ( B ) 1 ug/mL CRP (collagen-related peptide) to induce aggregation. C and D , Washed mouse platelets were treated with DMSO control (ctrl) or the Syk inhibitor piceatannol (15 umol/L) and stimulated with ( C ) 0.025 U/mL thrombin or ( D ) 1 ug/mL CRP to induce aggregation. After 2 min, platelets were lysed and analyzed via SDS-PAGE and Western Blot for phosphorylated p47phox at Ser 304 (p-p47phox S 304 ) or Ser 328 (p-p47phox S 328 ), total p47phox or alpha-tubulin as loading control. The blots were scanned and quantified using National Institutes of Health Image J and plotted as mean+-SEM (* P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA using a Phospho-p47phox/NCF1 pSer328 polyclonal antibody (Product # PA5-38777) comparing an epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings were taken at 450nM.
