Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R35891 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- p47phox Antibody / NCF1
- Antibody type
- Polyclonal
- Description
- This highly specific p47phox antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with human and mouse, pig samples.
- Reactivity
- Human, Mouse, Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the p47phox antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of U937 lysate with p47phox antibody at 0.2ug/ml. Expected/observed molecular weight ~47kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse thymus (A) and pig spleen (B) lysate with p47phox antibody at 1ug/ml. Expected/observed molecular weight ~47kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human colon tissue with p47phox antibody at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.