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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- MA5-14778 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p47phox Monoclonal Antibody (G.207.2)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- G.207.2
- Vial size
- 100 µL
- Concentration
- 35 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p47phox in extracts from Ramos and RL cells using p47phox monoclonal antibody (Product # MA5-14778).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-p47phox Monoclonal Antibody (G.207.2) (Product # MA5-14778) and a 50 kDa band corresponding to p47phox was observed in Raji and Daudi but was absent in A-431 and PC-3 which is reported to be low. Membrane enriched extracts (30 µg lysate) of Raji (Lane 1), Daudi (Lane 2), A-431 (Lane 3) and PC-3 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
