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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-34775 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cdc20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, THP-1. Predicted reactivity: Mouse (94%), Rat (94%), Pig (97%), Rhesus Monkey (99%), Chimpanzee (100%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDC20 using 30 µg THP-1 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CDC20 polyclonal antibody (Product # PA5-34775) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Cdc20 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cdc20 Polyclonal Antibody (Product # PA5-34775) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cdc20 Polyclonal Antibody (Product # PA5-34775). Sample (30 µg of whole cell lysate). Lane A: Hela. 10% SDS PAGE. Cdc20 Polyclonal Antibody (Product # PA5-34775) diluted at 1:1,000.
