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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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- Product number
- NB100-59827 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-59827, RRID:AB_2074910
- Product name
- Rabbit Polyclonal Cdc20 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Myosin phosphatase-targeting subunit 1 controls chromatid segregation.
Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.
Matsumura F, Yamakita Y, Yamashiro S
The Journal of biological chemistry 2011 Mar 25;286(12):10825-33
The Journal of biological chemistry 2011 Mar 25;286(12):10825-33
Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.
Ahlskog JK, Björk JK, Elsing AN, Aspelin C, Kallio M, Roos-Mattjus P, Sistonen L
Molecular and cellular biology 2010 Dec;30(24):5608-20
Molecular and cellular biology 2010 Dec;30(24):5608-20
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Cdc20 Antibody [NB100-59827] - Detection of Human CDC20 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC20 antibody NB100-59827 used for IP at 3 mcg/mg lysate. CDC20 was also immunoprecipitated by rabbit anti- CDC20 antibody NB100-59828, which recognizes a downstream epitope. The precipitates were blotted with NB100-59828.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Cdc20 Antibody [NB100-59827] - Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC20 antibody NB100-59827 (lot 2) used for IP at 6 ug/mg lysate. CDC20 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated CDC20, NB100-59827 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59827] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:500 (2ug/ml). Detection: DAB.
