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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- TA327982 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal anti-Acetylated Lysine Antibody
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal anti-Acetylated Lysine Antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- KAT2B
- Isotype
- IgG
- Antibody clone number
- 15G10
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Untreated Hela cells (lane 1) and sodium butyrate-treated Hela cells (24 hr treatment, lane 2) were lysed and cell extracts resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetylated lysine antibody (clone 15G10). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Untreated Hela cells (Upper Panel), or overnight nocodazole treated Hela cells (Lower Panel) stained with purified mouse monoclonal antibody against Acetylated Lysine (clone 15G10), followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
- Validation comment
- IF
