Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00008850-M06 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00008850-M06, RRID:AB_733235
- Product name
- PCAF monoclonal antibody (M06), clone 5E5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant PCAF.
- Antigen sequence
LEEEVYSQNSPIWDQDFLSASSRTSQLGIQTVINP
PPVAGTISYNSTSSSLEQPNAGSSSPACKASSGLE
ANPGEKRKMTDSHVLEEAKKPRVMGDIPME- Isotype
- IgG
- Antibody clone number
- 5E5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PCAF monoclonal antibody (M06), clone 5E5. Western Blot analysis of PCAF expression in C32 ( Cat # L002V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PCAF monoclonal antibody (M06), clone 5E5. Western Blot analysis of PCAF expression in human uterus myoma.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PCAF monoclonal antibody (M06), clone 5E5. Western Blot analysis of PCAF expression in Raw 264.7(Cat # L024V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PCAF is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PCAF on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol