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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- PA5-22046 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCAF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, A375, HeLa, HeLa nuclear extract, MFB. Predicted reactivity: Mouse (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCAF using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PCAF polyclonal antibody (Product # PA5-22046) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCAF was performed by separating 30 µg of HeLa whole cell and nuclear extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PCAF Polyclonal Antibody (Product # PA5-22046) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PCAF Polyclonal Antibody (Product # PA5-22046). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. PCAF Polyclonal Antibody (Product # PA5-22046) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCAF was performed by separating 30 µg of various whole cell lysates by 7.5 % SDS-PAGE. Proteins were transferred to a membrane and probed with a PCAF Polyclonal Antibody (Product # PA5-22046) at a dilution of 1:1000. A. 293T, B. A431, C. A375.
