GTX12188

antibody from GeneTex

Targeting: KAT2B GCN5, GCN5L, P/CAF, PCAF

Immunoprecipitation

Antibody data

Submit

Validation data

KAT2B protein structure - GTX12188 shown in red.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Recombinant human PCAF was separated on SDS-PAGE, blotted with Anti-PCAF (GTX12188) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody 2 μg/mL 2. Negative control: without antibody

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Nuclear extract of HeLa (lanes 1-2) and lysates of HepG2 (lanes 3-4) and Jurkat (lanes 5-6) cells were separated on SDS-PAGE, blotted with Anti-PCAF (817-832) (GTX12188) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1,3,5: Antibody 2 μg/mL 2,4,6: Antibody 2 μg/mL + PCAF immunizing peptide (human, 817-832)

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Lysate of Y79 cells was separated on SDS-PAGE, blotted with Anti-PCAF (GTX12188) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody 2 μg/mL 2. Antibody 2 μg/mL + PCAF immunizing peptide (human, 817-832)

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Rabbit Anti-PCAF (GTX12188) was used to immunoprecipitate PCAF from an exctract of Jurkat cells. Detection antibody: Rabbit Anti-PCAF (C-terminal). Lanes 1. 5 μg/mL antibody 2. Negative control: without antibody 3. 10 μg/mL antibody 4. Negative control: without cell extract

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HeLa whole cell and nuclear extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term (GTX12188) diluted at 1:1000.