10495-1-AP

antibody from Proteintech Group

Targeting: PIN1 dod

Provider product page for 10495-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [47]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 10495-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10495-1-AP, RRID:AB_2163943
Product name: PIN1 antibody
Antibody type: Polyclonal
Description: KD/KO validated PIN1 antibody (Cat. #10495-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PIN1 protein structure - 10495-1-AP shown in red.

Submitted references Identification and characterization of peptidyl-prolyl isomerase Pin1 as a new regulatory component of BCR::ABL1 degradation.

Tsukahara F, Kaji K, Maru Y
Leukemia research 2026 Jan;160:108146

DDX49 as a novel prognostic biomarker regulates colorectal cancer cell proliferation through the TIMM44-PI3K-AKT pathway.

Huang B, Yin Z, Gao G, Li L, Yang M
Frontiers in oncology 2025;15:1707092

Conserved mode of nuclear lamina distortion by primate cytomegaloviruses: importance of the pSer22 motif, viral kinase and cis/trans isomerase Pin1 activity.

Dhotre K, Schütz M, von Essen S, Fortelny L, Wangen C, Hahn F, Sticht H, Marschall M
The Journal of general virology 2025 Oct;106(10)

Ginsenoside Rh2 as a novel PIN1 inhibitor disrupting the cancer stem cell-like phenotype in non-small cell lung cancer.

Liu X, Mao Z, Yang J, Zhu Q, Dong X, Huang G, Shen X, Zhang L, Yang H
Journal of translational medicine 2025 Nov 10;23(1):1256

In vivo metabolic effects of naringin in reducing oxidative stress and protecting the vascular endothelium in dyslipidemic mice.

Zhang R, Wu S, Ye C, Li P, Xu B, Wang Y, Yang Z, Chen X, Chen J
The Journal of nutritional biochemistry 2025 May;139:109866

Covalent Inhibition of the Peptidyl-Prolyl Isomerase Pin1 by Sulfopin Results in a Broad Impact on the Phosphoproteome of Human Osteosarcoma U2-OS Cells.

Roffey SE, Hovey O, Jurcic K, Lu KP, Zhou XZ, Litchfield DW
Proteomics 2025 Jul;25(14):e13980

USP34 regulates PIN1-cGAS-STING axis-dependent ferroptosis in cervical cancer via SUMOylation.

Liao D, Cui Y, Shi L, Zeng S, Wang H
International immunopharmacology 2025 Feb 6;147:113968

CpG-Based Nanovaccines Enhance Ovarian Cancer Immune Response by Gbp2-Mediated Remodeling of Tumor-Associated Macrophages.

Xiong J, Huang J, Xu H, Wu Q, Zhao J, Chen Y, Fan G, Guan H, Xiao R, He Z, Wu S, Ouyang W, Wang S, Zhang L, Xia P, Zhang W, Wu M
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Apr;12(15):e2412881

Prolyl isomerase Pin1 in skeletal muscles contributes to systemic energy metabolism and exercise capacity through regulating SERCA activity.

Nakatsu Y, Matsunaga Y, Nakanishi M, Yamamotoya T, Sano T, Kanematsu T, Asano T
Biochemical and biophysical research communications 2024 Jun 30;715:150001

Cognitive integrity in Non-Demented Individuals with Alzheimer's Neuropathology is associated with preservation and remodeling of dendritic spines.

Guptarak J, Scaduto P, Tumurbaatar B, Zhang WR, Jupiter D, Taglialatela G, Fracassi A
Alzheimer's & dementia : the journal of the Alzheimer's Association 2024 Jul;20(7):4677-4691

Pin1 maintains the effector program of pathogenic Th17 cells in autoimmune neuroinflammation.

Fan G, Li G, Li L, Da Y
Journal of autoimmunity 2024 Jul;147:103262

The potential role of the prolyl isomerase Pin1 during blastocyst implantation and uterine decidualization in mice.

Xu R, Li Y, Li S, Wang N, Lin S, Du T, Wang F, Zhang X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jul 31;38(14):e23839

PIN1P1 is activated by CREB1 and promotes gastric cancer progression via interacting with YBX1 and upregulating PIN1.

Wang YW, Zhu WJ, Ma RR, Tian YR, Chen X, Gao P
Journal of cellular and molecular medicine 2024 Jan;28(1):e18022

Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells.

Zhu Q, Liang P, Meng H, Li F, Miao W, Chu C, Wang W, Li D, Chen C, Shi Y, Yu X, Ping Y, Niu C, Wu HB, Zhang A, Bian XW, Zhou W
Nature communications 2024 Jan 2;15(1):40

Akkermansia muciniphila Is Beneficial to a Mouse Model of Parkinson's Disease, via Alleviated Neuroinflammation and Promoted Neurogenesis, with Involvement of SCFAs.

Qiao CM, Huang WY, Zhou Y, Quan W, Niu GY, Li T, Zhang MX, Wu J, Zhao LP, Zhao WJ, Cui C, Shen YQ
Brain sciences 2024 Feb 29;14(3)

Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy.

Fang Y, Kang Z, Zhang W, Xiang Y, Cheng X, Gui M, Fang D
BMC pregnancy and childbirth 2024 Aug 10;24(1):525

KPT6566 induces apoptotic cell death and suppresses the tumorigenicity of testicular germ cell tumors.

Sun R, Lee EJ, Lee S, Kim G, Kim J
Frontiers in cell and developmental biology 2023;11:1220179

Nanocarrier of Pin1 inhibitor based on supercritical fluid technology inhibits cancer metastasis by blocking multiple signaling pathways.

Zhang F, Zhang A, Xie Y, Wen H, Kankala RK, Huang J, Zhang A, Wang Q, Chen B, Dong H, Guo Z, Chen A, Yang D
Regenerative biomaterials 2023;10:rbad014

MicroRNA-150-5p inhibits the proliferation and invasion of human larynx epidermiod cancer cells though regulating peptidyl-prolyl cis/trans isomerase.

Chen H, Cai X, Du B, Cai J, Luo Z
Brazilian journal of otorhinolaryngology 2023 May-Jun;89(3):383-392

Hepatic Pin1 Expression, Particularly in Nuclei, Is Increased in NASH Patients in Accordance with Evidence of the Role of Pin1 in Lipid Accumulation Shown in Hepatoma Cell Lines.

Kanna M, Nakatsu Y, Yamamotoya T, Kushiyama A, Fujishiro M, Sakoda H, Ono H, Arihiro K, Asano T
International journal of molecular sciences 2023 May 16;24(10)

Mechanical Regulation of Redox Balance via the Induction of the PIN1/NRF2/ARE Axis in Pancreatic Cancer.

Liang C, Rong Z, Tuerhong A, Meng Q, Hua J, Liu J, Zhang B, Wang W, Yu X, Shi S, Xu J
International journal of molecular sciences 2023 Feb 9;24(4)

Upregulation of Pin1 contributes to alleviation of cognitive dysfunction in diabetic mice.

Qiu D, Liu T, Qianping W
Brain and behavior 2023 Dec;13(12):e3336

The expression of prolyl isomerase Pin1 is expanded in the skin of patients with atopic dermatitis and facilitates IL-33 expression in HaCaT cells.

Kanamoto M, Takahagi S, Aoyama S, Kido Y, Nakanishi M, Naito M, Kanna M, Yamamotoya T, Tanaka A, Hide M, Asano T, Nakatsu Y
The Journal of dermatology 2023 Apr;50(4):462-471

PIN1 and CDK1 cooperatively govern pVHL stability and suppressive functions.

Chen J, Li M, Liu Y, Guan T, Yang X, Wen Y, Zhu Y, Xiao Z, Shen X, Zhang H, Tang H, Liu T
Cell death and differentiation 2023 Apr;30(4):1082-1095

Prolyl isomerase Pin1 promotes extracellular matrix production in hepatic stellate cells through regulating formation of the Smad3-TAZ complex.

Aoyama S, Kido Y, Kanamoto M, Naito M, Nakanishi M, Kanna M, Yamamotoya T, Asano T, Nakatsu Y
Experimental cell research 2023 Apr 15;425(2):113544

Juglone and KPT6566 Suppress the Tumorigenic Potential of CD44(+)CD133(+) Tumor-Initiating Caco-2 Cells In Vitro and In Vivo.

Kim J, Lee S, Sun R, Kim J
Frontiers in cell and developmental biology 2022;10:861045

Disturbing NLRP3 acetylation and inflammasome assembly inhibits androgen receptor-promoted inflammatory responses and prostate cancer progression.

Zhao AN, Yang Z, Wang DD, Shi B, Zhang H, Bai Y, Yan BW, Zhang Y, Wen JK, Wang XL, Qu CB
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 Nov;36(11):e22602

Pin1-mediated regulation of articular cartilage stem/progenitor cell aging.

Zhang X, Sun W, Wu W, Chen M, Ji T, Xu H, Wang Y
Tissue & cell 2022 Jun;76:101765

PIN1-mediated ROS production is involved in antagonism of N-acetyl-L-cysteine against arsenic-induced hepatotoxicity.

Zhang H, He Z, Deng P, Lu M, Zhou C, Yang L, Yu Z
Toxicology research 2022 Aug;11(4):628-643

Inhibition of the PIN1-NRF2/GPX4 axis imparts sensitivity to cisplatin in cervical cancer cells.

Zhang Z, Hu Q, Ye S, Xiang L
Acta biochimica et biophysica Sinica 2022 Aug 25;54(9):1325-1335

PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.

Zhang Y, Lv Z, Liu Y, Cao H, Yang J, Wang B
Oxidative medicine and cellular longevity 2021;2021:9980444

Pin1 Promotes NLRP3 Inflammasome Activation by Phosphorylation of p38 MAPK Pathway in Septic Shock.

Dong R, Xue Z, Fan G, Zhang N, Wang C, Li G, Da Y
Frontiers in immunology 2021;12:620238

SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.

Lin L, Chen S, Wang H, Gao B, Kallakury B, Bhuvaneshwar K, Cahn K, Gusev Y, Wang X, Wu Y, Marshall JL, Zhi X, He AR
Theranostics 2021;11(9):4232-4250

Cobalt induces neurodegenerative damages through Pin1 inactivation in mice and human neuroglioma cells.

Zheng F, Li Y, Zhang F, Sun Y, Zheng C, Luo Z, Wang YL, Aschner M, Zheng H, Lin L, Cai P, Shao W, Guo Z, Zheng M, Zhou XZ, Lu KP, Wu S, Li H
Journal of hazardous materials 2021 Oct 5;419:126378

Phenotypical Characterization of the Nuclear Egress of Recombinant Cytomegaloviruses Reveals Defective Replication upon ORF-UL50 Deletion but Not pUL50 Phosphosite Mutation.

Häge S, Sonntag E, Svrlanska A, Borst EM, Stilp AC, Horsch D, Müller R, Kropff B, Milbradt J, Stamminger T, Schlötzer-Schrehardt U, Marschall M
Viruses 2021 Jan 22;13(2)

Prolyl isomerase Pin1 interacts with adipose triglyceride lipase and negatively controls both its expression and lipolysis.

Nakatsu Y, Yamamotoya T, Okumura M, Ishii T, Kanamoto M, Naito M, Nakanishi M, Aoyama S, Matsunaga Y, Kushiyama A, Sakoda H, Fujishiro M, Ono H, Asano T
Metabolism: clinical and experimental 2021 Feb;115:154459

LGR5 induces β-catenin activation and augments tumour progression by activating STAT3 in human intrahepatic cholangiocarcinoma.

Kawasaki K, Kuboki S, Furukawa K, Takayashiki T, Takano S, Ohtsuka M
Liver international : official journal of the International Association for the Study of the Liver 2021 Apr;41(4):865-881

Melatonin directly binds and inhibits death-associated protein kinase 1 function in Alzheimer's disease.

Chen D, Mei Y, Kim N, Lan G, Gan CL, Fan F, Zhang T, Xia Y, Wang L, Lin C, Ke F, Zhou XZ, Lu KP, Lee TH
Journal of pineal research 2020 Sep;69(2):e12665

Halo and Pseudohalo Gold(I)-NHC Complexes Derived from 4,5-Diarylimidazoles with Excellent In Vitro and In Vivo Anticancer Activities Against HCC.

Bian M, Fan R, Jiang G, Wang Y, Lu Y, Liu W
Journal of medicinal chemistry 2020 Sep 10;63(17):9197-9211

Deficiency of microRNA-628-5p promotes the progression of gastric cancer by upregulating PIN1.

Chen Y, Wu Y, Yu S, Yang H, Wang X, Zhang Y, Zhu S, Jie M, Liu C, Li X, Zhou Y, Yang S, Yang Y
Cell death & disease 2020 Jul 23;11(7):559

The peptidyl-prolyl cis/trans isomerase Pin1 interacts with three early regulatory proteins of human cytomegalovirus.

Schütz M, Thomas M, Wangen C, Wagner S, Rauschert L, Errerd T, Kießling M, Sticht H, Milbradt J, Marschall M
Virus research 2020 Aug;285:198023

TGF-β1-PML SUMOylation-peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) form a positive feedback loop to regulate cardiac fibrosis.

Wu D, Huang D, Li LL, Ni P, Li XX, Wang B, Han YN, Shao XQ, Zhao D, Chu WF, Li BY
Journal of cellular physiology 2019 May;234(5):6263-6273

Cellular glucose metabolism is essential for the reduction of cell-impermeable water-soluble tetrazolium (WST) dyes.

Xie L, Dai Z, Pang C, Lin D, Zheng M
International journal of biological sciences 2018;14(11):1535-1544

Pin1 Promotes Regulated Necrosis Induced by Glutamate in Rat Retinal Neurons via CAST/Calpain2 Pathway.

Wang S, Liao L, Wang M, Zhou H, Huang Y, Wang Z, Chen D, Ji D, Xia X, Wang Y, Liu F, Huang J, Xiong K
Frontiers in cellular neuroscience 2017;11:425

The Essential Role of Pin1 via NF-κB Signaling in Vascular Inflammation and Atherosclerosis in ApoE(-/-) Mice.

Liu M, Yu P, Jiang H, Yang X, Zhao J, Zou Y, Ge J
International journal of molecular sciences 2017 Mar 16;18(3)

Down-regulation of Pin1 in Temporal Lobe Epilepsy Patients and Mouse Model.

Tang L, Zhang Y, Chen G, Xiong Y, Wang X, Zhu B
Neurochemical research 2017 Apr;42(4):1211-1218

Pin1 promotes GR transactivation by enhancing recruitment to target genes.

Poolman TM, Farrow SN, Matthews L, Loudon AS, Ray DW
Nucleic acids research 2013 Oct;41(18):8515-25

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10495-1-AP(PIN1 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The PIN1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human PIN1. This antibody recognizes human,mouse,rat antigen. The PIN1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 10495-1-AP.pdf

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