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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-35372 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with non-human primate based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PIN1 in 293 cells (right) compared to a negative control (left) using a PIN1 polyclonal antibody (Product # PA5-35372) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PIN1 in 293 cells. Samples were incubated in PIN1 polyclonal antibody (Product # PA5-35372) followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PIN1 in formalin-fixed and paraffin-embedded human lung carcinoma. Samples were incubated with PIN1 polyclonal antibody (Product # PA5-35372) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of PIN1 in MCF-7 cells (bottom) compared to a negative control (top) using a PIN1 polyclonal antibody (Product # PA5-35372) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of PIN1 in MCF-7 cells (bottom histogram). Samples were incubated with PIN1 polyclonal antibody (Product # PA5-35372) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).
