Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PAB11244 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11244, RRID:AB_1711115
- Product name
- PIN1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PIN1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Aberrant expression of beta-catenin, Pin1 and cylin D1 in salivary adenoid cystic carcinoma: relation to tumor proliferation and metastasis.
The peptidyl-prolyl isomerase Pin1 regulates granulocyte-macrophage colony-stimulating factor mRNA stability in T lymphocytes.
Prolyl isomerase Pin1 expression predicts prognosis in patients with esophageal squamous cell carcinoma and correlates with cyclinD1 expression.
Zhou CX, Gao Y
Oncology reports 2006 Sep;16(3):505-11
Oncology reports 2006 Sep;16(3):505-11
The peptidyl-prolyl isomerase Pin1 regulates granulocyte-macrophage colony-stimulating factor mRNA stability in T lymphocytes.
Esnault S, Shen ZJ, Whitesel E, Malter JS
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):6999-7006
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):6999-7006
Prolyl isomerase Pin1 expression predicts prognosis in patients with esophageal squamous cell carcinoma and correlates with cyclinD1 expression.
Fukuchi M, Fukai Y, Kimura H, Sohda M, Miyazaki T, Nakajima M, Masuda N, Tsukada K, Kato H, Kuwano H
International journal of oncology 2006 Aug;29(2):329-34
International journal of oncology 2006 Aug;29(2):329-34
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using PIN1 polyclonal antibody (Cat # PAB11244) to detect endogenous PIN1 in HeLa whole cell lysates.The sample was run in duplicate.A band representing PIN1 is indicated by the arrowhead.Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose.The membrane was probed with the primary antibody at a 1 : 700 dilution.A 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG was used with a 15 sec exposure time.Personal Communication, L. D'agostino and A. Giordano, SHRO, Philadelphia, PA.