Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-77757 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77757, RRID:AB_11033967
- Product name
- Rabbit Polyclonal Pin1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. A BLAST analysis was used to suggest cross-reactivity with Pin1 from human, dog, bovine and monkey based on a 100% homology with the immunizing sequence. Expect partial reactivity with Pin1 from mouse and rat sources based on 92% sequence homologies. Reactivity against homologues from other sources is not known.
- Reactivity
- Human, Bovine, Canine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pin1 Antibody [NBP1-77757] - Lane 1: T9ug cells treated with scrambled (scr). Lane 2: T9ug cells treated with PIN1 kd1 shRNA. Lane 3: T9ug cells treated with PIN1-overexpressing plasmid HAP1. Lane 4: T9ug cells treated with PIN1-overexpressing plasmid HAP1S67E. Lane 5: T9ug cells treated with PIN1 kd2 shRNA. Load: 25 ug per lane. Primary antibody: PIN 1 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted size: 18 kDa for PIN-1. Other band(s): normalized with a-tubulin (a-tub) antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pin1 Antibody [NBP1-77757] - Detection of endogenous Pin1 in HeLa whole cell lysates. The sample was run in duplicate. A band representing Pin1 is indicated by the arrowhead. Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose. The membrane was probed with the primary antibody at a 1:700 dilution. A 1:5,000 dilution of HRP Gt-a-Rabbit IgG was used with a 15 sec exposure time.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pin1 Antibody [NBP1-77757] - Lane 1: T9ug cells incubated with GST-Pin1. Lane 2: T9ug cells incubated with GST-CDK4/cyclinD1. Lane 3: T9ug cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 ug per lane. Primary antibody: anti-GST 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Pin1 Antibody [NBP1-77757] - Tissue: testis. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-PIN1 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PIN-1 as precipitated red signal with hematoxylin purple nuclear counterstain.