NBP1-77757

antibody from Novus Biologicals

Targeting: PIN1 dod

ELISA

Immunoprecipitation

Antibody data

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Validation data

Product number: NBP1-77757 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP1-77757, RRID:AB_11033967
Product name: Rabbit Polyclonal Pin1 Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified. A BLAST analysis was used to suggest cross-reactivity with Pin1 from human, dog, bovine and monkey based on a 100% homology with the immunizing sequence. Expect partial reactivity with Pin1 from mouse and rat sources based on 92% sequence homologies. Reactivity against homologues from other sources is not known.
Reactivity: Human, Bovine, Canine, Simian
Host: Rabbit
Isotype: IgG
Vial size: 0.1 mg
Concentration: 1.1 mg/ml
Storage: Store at -20C. Avoid freeze-thaw cycles.

PIN1 protein structure - NBP1-77757 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Pin1 Antibody [NBP1-77757] - Lane 1: T9ug cells treated with scrambled (scr). Lane 2: T9ug cells treated with PIN1 kd1 shRNA. Lane 3: T9ug cells treated with PIN1-overexpressing plasmid HAP1. Lane 4: T9ug cells treated with PIN1-overexpressing plasmid HAP1S67E. Lane 5: T9ug cells treated with PIN1 kd2 shRNA. Load: 25 ug per lane. Primary antibody: PIN 1 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted size: 18 kDa for PIN-1. Other band(s): normalized with a-tubulin (a-tub) antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Pin1 Antibody [NBP1-77757] - Detection of endogenous Pin1 in HeLa whole cell lysates. The sample was run in duplicate. A band representing Pin1 is indicated by the arrowhead. Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose. The membrane was probed with the primary antibody at a 1:700 dilution. A 1:5,000 dilution of HRP Gt-a-Rabbit IgG was used with a 15 sec exposure time.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Pin1 Antibody [NBP1-77757] - Lane 1: T9ug cells incubated with GST-Pin1. Lane 2: T9ug cells incubated with GST-CDK4/cyclinD1. Lane 3: T9ug cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 ug per lane. Primary antibody: anti-GST 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.