PA5-29675

antibody from Invitrogen Antibodies

Targeting: PIN1 dod

Antibody data

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Validation data

Product number: PA5-29675 - Provider product page
Provider: Invitrogen Antibodies
Product name: PIN1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (94%), Rat (95%), Xenopus laevis (85%), Pig (97%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.68 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PIN1 protein structure - PA5-29675 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PIN1 Polyclonal Antibody (Product # PA5-29675). Sample (30 µg of whole cell lysate). Lane A: Hep G2. Lane B: Raji. 12% SDS PAGE. PIN1 Polyclonal Antibody (Product # PA5-29675) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PIN1 antibody detects PIN1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg Raw264.7 whole cell lysate/extract. G. 30 µg C2C12 whole cell lysate/extract.15 % SDS-PAGE. PIN1 antibody PIN1 Polyclonal Antibody (Product # PA5-29675) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PIN1 antibody detects PIN1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.15 % SDS-PAGE. PIN1 antibody PIN1 Polyclonal Antibody (Product # PA5-29675) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti- PIN1 Polyclonal Antibody (Product # PA5-29675) and a 18 kDa band corresponding to PIN1 was observed across the cell lines tested. An additional uncharacterized band (*) was observed around 30 kDa. Whole cell extracts (1%SDS) (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2) and Neuro 2a (Lane 3) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).