Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS4156 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS4156, RRID:AB_1663854
- Product name
- PKC ø (phospho T410) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human PKC ø around the phosphorylation site of Threonine 410.
- Description
- Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (Ï«, ÉÇI, ³Úii and ÎÛ) and novel (n) PKC isoforms (Ñ´, ÷ , ø, »Ò and IJ). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC Ñ´ and ÷ are independent of Ca++. On the other hand, nPKC Ñ´ and ÷, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-PKC ¦Æ (T410) pAb at 1:500 dilutionLane1:MCF-7 cell lysate treated with TNFaLane2:NIH-3T3 cell lysate treated with LPSLane3:PC12 cell lysate treated with UV