Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- Ab122119 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- PKC zeta Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human PKC zeta Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 0.01-2 µg/mL IHC: 5-20 µg/mL Protein Function This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 404-591- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
No comments: Submit comment
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- PKC zeta Antibody (Ab122119) - Western Blot All lanes: PKC zeta Antibody (Ab122119) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 68 kDa Observed band size: 46, 71, 78 kDa Exposure time: 33.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- PKC zeta Antibody (Ab122119) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: 20 μg/mL PKC zeta Antibody (Ab122119) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
