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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-29337 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKC zeta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (91%), Pig (99%), Rabbit (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PKC zeta using 30 µg of A) HeLa and B) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PKC zeta polyclonal antibody (Product # PA5-29337) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PKC zeta was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKC zeta Polyclonal Antibody (Product # PA5-29337) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PKC zeta Polyclonal Antibody detects PKC zeta protein at cytoplasm on SW480 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SW480 xenograft. PKC zeta Polyclonal Antibody (Product # PA5-29337) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
