TA500769

antibody from OriGene

Targeting: PPME1 ABDH19, PME-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500769

Provider

OriGene

Proper citation

OriGene Cat#TA500769, RRID:AB_11126575

Product name

Anti-PPME1 mouse monoclonal antibody, clone OTI10B2 (formerly 10B2)

Antibody type

Monoclonal

Description

Anti-PPME1 mouse monoclonal antibody, clone OTI10B2 (formerly 10B2)

Host

Mouse

Conjugate

Unconjugated

Epitope

PPME1

Isotype

IgG

Antibody clone number

OTI10B2

Vial size

100 µl

Concentration

0.87 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPME1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from a mouse tissue by using anti-PPME1 monoclonal antibody.(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PPME1 mouse monoclonal antibody (TA500769) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1(RC200009).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either pCMV6-ENTRY PPME1(RC200009)(Red) or empty vector control plasmid(Blue) were immunostained with anti-PPME1 mouse monoclonal(TA500769, Dilution 1:1,000), and then analyzed by flow cytometry.

Validation comment

FC