TA500840

antibody from OriGene

Targeting: PPME1 ABDH19, PME-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500840

Provider

OriGene

Proper citation

OriGene Cat#TA500840, RRID:AB_11126052

Product name

Anti-PPME1 mouse monoclonal antibody, clone OTI6H5 (formerly 6H5)

Antibody type

Monoclonal

Description

Anti-PPME1 mouse monoclonal antibody, clone OTI6H5 (formerly 6H5)

Host

Mouse

Conjugate

Unconjugated

Epitope

PPME1

Isotype

IgG

Antibody clone number

OTI6H5

Vial size

100 µl

Concentration

0.83 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PPME1 mouse monoclonal antibody (TA500840) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1(RC200009).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500840, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500840, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500840, Dilution 1:50)

Validation comment

IHC