Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-B9567 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ MAPKAPK5 / PRAK Antibody (aa306-318) LS-B9567
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Horse, Porcine, Rabbit, Simian
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- MAPKAPK5 antibody and EB11928 (1.5 ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with mouse anti-MK5 / PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- MAPKAPK5 antibody HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with (0.5 ug/ml) in right panel and with EB11928 (0.5 ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.