LS-B9569

antibody from LSBio

Targeting: MAPKAPK5 MK5, PRAK

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B9569

Provider

LSBio

Product name

IHC-plus™ MAPKAPK5 / PRAK Antibody (aa192-206) LS-B9569

Antibody type

Polyclonal

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Horse, Porcine, Rabbit, Simian, Zebrafish

Host

Goat

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MAPKAPK5 antibody EB11927 and (1.5 ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with mouse anti-MK5 / PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MAPKAPK5 antibody HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with EB11927 (0.5 ug/ml) in right panel and with (0.5 ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.