AP32808PU-N

antibody from Acris Antibodies GmbH

Targeting: MAPKAPK5 MK5, PRAK

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP32808PU-N

Provider

Acris Antibodies GmbH

Product name

anti MAPKAP Kinase-5

Antibody type

Polyclonal

Antigen

Peptide with sequence from the internal region of the protein sequence according to NP_003659.2; NP_620777.1.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Porcine

Host

Goat

Vial size

0.1 mg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with AP32808PU-N (0.5 µg/ml) in right panel and with AP32809PU-N (0.5 µg/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.

Immunoprecipitation

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP32808PU-N and AP32808PU-N (1.5 µg) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with mouse anti-MK5 / PRAK (and co-precipitation was measured using Rabbit anti-ERK3 in the lower panel).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP32808PU-N (5 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.