Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP32809PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti MAPKAP Kinase-5 (192-206)
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence from the internal region (192-206) of the protein sequence according to NP_003659.2; NP_620777.1.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HEK293 lysate (10ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with AP32808PU-N (0.5 μg/ml) in right panel and with AP32809PU-N (0.5ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP32808PU-N andAP32808PU-N (1.5 μg) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with mouse anti-MK5 / PRAK(and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP32809PU-N (5 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.