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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- R35126 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PRAK Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific PRAK antibody is suitable for use in Western blot/ELISA/Immunoprecipitation/Immunohistochemistry applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the PRAK antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of HEK293 lysate overexpressing mouse MK5/PRAK (first lane) or mouse MK2 (second lane) tested with right ) PRAK antibody (cat # R36080, 0.5ug/ml) and left) PRAK antibody (cat # R35126, 0.5ug/ml). No cross-reaction seen with MK2 protein.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IP: PRAK antibody (cat # R36080 and R35126) used at 1.5ug for immunoprecipitation from lysates of PRAK/ERK3 double knockout mouse embryonic firbroblasts, with (third and fifth lanes) and without (fourth and sixth lanes) rescued PRAK/ERK3 expression through retroviral transduction.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human kidney with PRAK antibody at 5ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
