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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- AP32808PU-N - Provider product page
- Provider
- OriGene
- Product name
- PRAK (MAPKAPK5) goat polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- PRAK (MAPKAPK5) goat polyclonal antibody, Aff - Purified
- Host
- Goat
- Conjugate
- Unconjugated
- Epitope
- MAPKAPK5
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with ?(0.5 ug/ml) in right panel and with -N?(0.5 ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ?and (1.5 ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with mouse anti-MK5 / PRAK (and co-precipitation was measured using Rabbit anti-ERK3 in the lower panel).
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ?(5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- IHC
