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- Antibody Data
- Antigen structure
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- Validations
- Other assay [2]
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- Product number
- STJ72940 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-MK5/MAPKAPK5 antibody (Internal) (STJ72940)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-MK5/MAPKAPK5 (Internal) is suitable for use in ELISA and Western Blot research applications.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
STEALDNVLPSAQ- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
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Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- HEK293 lysate (10ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with STJ72940 (0. 5ug/ml) in right panel and with STJ72941 (0. 5ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ72940 and STJ72941 (1. 5ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp. ) were analyzed in parallel in this Western blot labelled with mouse anti-MK5/ PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
